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Part:BBa_M36192:Design

Designed by: Everett Frost   Group: Stanford BIOE44 - S11   (2012-06-03)

Bicistronic Linker Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a spacer sequence designed to fit between two gene coding regions and prevent RBS's from interfering with each other. We used it in part Bba_M36196. This part is 106 base pairs of alpha helix coding areas interspersed with TAA stop codons.

Design Notes There was some difficulty in synthesizing this part due to the number of TAA repeats. A prokaryotic ribosome is about 200 angstroms in length, and a typical base pair is 3.4 angstroms. This comes to a minimum of 58 nucleotides necessary to separate ribosomes ending translation of one gene and beginning translation of the next from interacting or preventing each other's activity. We designed this part to be over 100 bp in length to circumvent this problem.


Source

This part is custom designed using a generic genetic engineering program.

Aaron Thayer and Everett Frost custom designed this part.

References